Coding

Part:BBa_K2833018:Design

Designed by: Wanji Li   Group: iGEM18_BJRS_China   (2018-10-04)


J23104+B0032+mEos+autotranspoter+Ter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 872
    Illegal XbaI site found at 899
    Illegal PstI site found at 911
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 872
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 911
    Illegal NotI site found at 864
    Illegal NotI site found at 929
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 872
    Illegal BamHI site found at 893
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 872
    Illegal XbaI site found at 899
    Illegal PstI site found at 911
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 872
    Illegal XbaI site found at 899
    Illegal PstI site found at 911
    Illegal NgoMIV site found at 794
    Illegal NgoMIV site found at 1731
    Illegal AgeI site found at 1506
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

Parts are all from Guoqiang Chen lab.

References